史珊怡;邢菁;陈奥;武良群;马伯艳;郭文海;徐佳;

• 1:黑龙江中医药大学附属第二医院
• 2:黑龙江中医药大学

摘要(Abstract)：

目的 观察“原络通经”针刺法对SAMP8小鼠学习记忆能力的影响，探究“原络通经”针刺法促进痴呆小鼠学习记忆能力恢复的作用机制。方法 选取SAMR1小鼠6只作为SAMR1组，常规饲养，仅每天抓取；另选取SAMP8小鼠18只，采用随机数字表法分成SAMP8空白组、“原络通经”组和百会组各6只，SAMP8空白组仅每天抓取，“原络通经”组和百会组给予相应针刺干预。各组干预30 d后，采用Morris水迷宫实验检测小鼠学习记忆与空间探索能力，HE染色观察小鼠海马区神经细胞变化，Western blot法检测海马组织中N-甲基-D-天冬氨酸受体(NMDAR)表达情况，ELISA法检测小鼠海马组织中Aβ_(40)蛋白含量。结果 与SAMP1组比较，SAMP8空白组小鼠逃避潜伏期明显延长，原平台象限停留时间明显缩短，穿过原平台位置次数明显减少，差异均有统计学意义(P均<0.05);与SAMP8空白组比较，“原络通经”组与百会组SAMP8小鼠逃避潜伏期均明显缩短，原平台象限停留时间明显延长，穿过原平台位置次数明显增加，且“原络通经”组改善情况均明显优于百会组，差异均有统计学意义(P均<0.05)。海马HE染色显示SAMR1组小鼠海马组织神经细胞形态、结构正常；SAMP8空白组小鼠海马组织神经细胞皱缩，数目减少，光度值明显低于SAMR1组(P<0.05);“原络通经”组与百会组小鼠海马组织神经细胞形态与数量较SAMP8空白组明显改善，光度值明显增高(P均<0.05),且“原络通经”组光度值明显高于百会组(P<0.05)。与SAMR1组比较，SAMP8空白组小鼠海马组织中NMDAR蛋白表达量明显降低(P<0.05),海马组织中Aβ_(40)蛋白含量明显增高(P<0.05);与SAMP8空白组比较，“原络通经”组与百会组小鼠海马组织中NMDAR蛋白表达量均明显升高(P均<0.05),海马组织中Aβ_(40)蛋白含量均明显降低(P均<0.05),且“原络通经”组与百会组比较改善更明显(P均<0.05)。结论 “原络通经”针刺与百会穴针刺治疗均可能通过促进海马组织中NMDAR表达、抑制Aβ_(40)沉积改善SAMP8小鼠的学习记忆能力，但“原络通经”针刺法效果优于百会穴针刺法。

关键词(KeyWords)： 痴呆;Aβ_(40);N-甲基-D-天冬氨酸受体;原络通经;针刺疗法;百会穴

Abstract:

Keywords:

基金项目(Foundation): 黑龙江省中医药科研项目(ZHY19-049);; 黑龙江省自然科学基金项目(H2018061)

作者(Authors): 史珊怡;邢菁;陈奥;武良群;马伯艳;郭文海;徐佳;

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