李慧堂;张岱男;张新中;金保哲;肖萌;李海明;

• 1:新乡医学院第一附属医院河南省神经修复重点实验室
• 2:首都医科大学附属北京天坛医院

摘要(Abstract)：

目的 探讨黄芪甲苷对大鼠创伤性脑损伤后脑水肿、炎症反应、细胞凋亡的影响。方法 将120只SD大鼠随机分为假手术组、模型组和黄芪甲苷组，每组40只。模型组和黄芪甲苷组运用Feeney DM法制作创伤性脑损伤模型，假手术组不进行击打。造模成功后，黄芪甲苷组给予黄芪甲苷20 mg/(kg·d)腹腔注射，假手术组和模型组每天给予等量0.9%NaCl腹腔注射直至处死。分别于造模后12 h、1 d、3 d、5 d、7 d进行改良大鼠神经功能缺损严重程度评分(mNSS)和脑组织含水量检测；取造模后3 d脑组织，HE及尼氏染色观察脑组织神经细胞病理形态学变化，TUNEL法检测损伤灶周围脑组织神经元细胞凋亡情况，ELISA法测定损伤灶及周围脑组织炎症因子白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量。结果 造模后12 h、1 d、3 d、5 d、7 d,模型组、黄芪甲苷组mNSS评分和脑组织含水量均明显高于同期假手术组(P均<0.05),且mNSS评分造模后12 h最高，脑组织含水量造模后3 d达到峰值，此后均逐渐降低；黄芪甲苷组造模后12 h mNSS评分和造模后12 h、1 d脑组织含水量与模型组比较差异均无统计学意义(P均>0.05);黄芪甲苷组造模后1 d、3 d、5 d、7 d mNSS评分及造模后3 d、5 d、7 d脑组织含水量均明显低于同期模型组(P均<0.05)。HE及尼氏染色显示模型组大鼠有明显脑组织细胞变性坏死、炎性细胞浸润等病变，神经元细胞大量减少、排列紊乱，尼氏体明显减少；与模型组比较，黄芪甲苷组神经元细胞和尼氏体增多，神经元损伤轻。造模3 d后，模型组和黄芪甲苷组大鼠脑组织神经细胞凋亡率、损伤灶及周围脑组织中TNF-α、IL-6、IL-1β含量均明显高于假手术组(P均<0.05),黄芪甲苷组均明显低于模型组(P均<0.05)。结论 黄芪甲苷可以通过减轻脑组织水肿及神经细胞损伤，抑制神经细胞凋亡及中枢性炎症反应而起到神经保护作用。

关键词(KeyWords)： 黄芪甲苷;创伤性颅脑损伤;脑水肿;炎症反应;细胞凋亡

Abstract:

Keywords:

基金项目(Foundation): 河南省科技发展计划项目(162102310120);; 河南省医学科技攻关计划项目(SBGJ2018057)

作者(Authors): 李慧堂;张岱男;张新中;金保哲;肖萌;李海明;

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